Isoelectric focusing (IEF) is an electrophoretic technique that separates amphoteric molecules (like proteins) based on their isoelectric point (pI), the pH at which their net charge is zero, by applying an electric field across a pH gradient.
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Overview
Features:
- Separation based on isoelectric points
- pH gradient stability monitoring
- High-resolution protein separation
- Minimal heat generation
- Compatible with 2D electrophoresis
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