The most basic electrophoresis system consists of a chamber or cell, a membrane, an incubator or oven, and a power supply. The chamber contains two buffer compartments, one at each end of the chamber. A bridge between the two compartments supports a membrane, typically cellulose acetate or some type of gel.
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Overview
Features:
- Power supply
- Provides an electric current to the chamber to separate charged particles. A good power supply offers precise AC to DC conversion, multiple operating modes, and abnormality alerts.
- Chamber
- Contains two buffer compartments, one at each end, and a membrane that supports the buffer. The chamber also has two electrodes, a cathode and an anode.
- Sample loading
- Samples are loaded into wells in the gel using a pipette.
- Separation
- Charged particles in the sample move through the gel towards the oppositely charged electrode. Smaller molecules move faster than larger molecules.
- Visualization
- The gel is stained to visualize the bands, or the DNA is transferred to a membrane.
- Size determination
- The size of the substance of interest is determined by comparing it to nucleic acid markers of known size.
